Apsilon

The Apsilon™ family of DNA/RNA extraction kits offers a fast, enzyme-free, alcohol-free, room-temperature-stable approach to nucleic acid purification across a sweeping range of sample types, including whole blood, swabs, tissues, fluids, and even challenging matrices like FFPE, stool, and soil. Designed for both manual and high-throughput automated workflows, Apsilon™ kits achieve extraction in 10–13 minutes without centrifugation, bead drying, or enzymatic pretreatment, yet deliver high yields and consistently strong performance in qPCR, RT-qPCR, LAMP, and NGS. With up to 90% recovery, minimal inhibition, and proven superiority or parity with leading commercial kits, the Apsilon™ system provides a single streamlined solution for pathogen detection, host nucleic acid extraction, and advanced sequencing applications — all supported by an ambient-temperature Molecular Transport Medium that inactivates pathogens while preserving DNA and RNA.

Core Technology

• Magnetic-bead extraction optimized for speed and purity.

• No enzymes required (no Proteinase K, no lysozyme).

• Alcohol-free formulation for safe shipping and consistent chemistry.

• Room-temperature stability — no refrigeration or cold chain needed.

• 10–13 minute extraction depending on kit.

• Manual or automated processing (compatible with KingFisher, M32, AutoPure Mini, etc.).

• No centrifugation, no bead drying, no pretreatment even for tough organisms.

Performance

• Up to 90% nucleic acid recovery.

• High-purity DNA/RNA with minimal PCR inhibition.

• Strong performance validated against major competitors (Q, M, N, T, K kits).

• Compatible with:

  • qPCR / RT-qPCR

  • Isothermal assays (LAMP/RT-LAMP)

  • NGS / metagenomic sequencing

• Demonstrated ability to extract host DNA/RNA + microbial nucleic acids simultaneously.

Sample Type Coverage

Apsilon™ kits support an unusually broad set of matrices:

• Whole blood, serum, plasma

• Respiratory, genito, wound, vaginal, rectal swabs (dry or UTM/VTM/MTM)

• Saliva, urine, stool

• Tissues, FFPE, cell pellets, culture media

• Nasal swabs

• Purified exosomes

• Soil and other complex environmental samples

• Pathogens:

  • DNA/RNA viruses

  • Gram-positive & gram-negative bacteria

  • Mycobacteria

  • Fungi

  • Host genomic DNA / RNA

Kit Portfolio Overview

• Universal DNA/RNA Extraction Kit

• Whole Blood DNA/RNA Extraction Kit (Host & Pathogen)

• Nasal Swab DNA/RNA Extraction Kit

• STD (Swab) DNA/RNA Extraction Kit

• Nasal Swab DNA-only Extraction Kit (in development)

• Formats: 24-prep, 96-prep, 480-prep

Workflow Advantages

• Simple 1-plate protocol for automation.

• Full extraction in 10–13 minutes (system or manual).

• Unified chemistry across sample types → simplified training & logistics.

• No hazardous materials, enabling easier shipping & storage.

• Reduced plastic usage (40% less) for lower environmental impact.

Molecular Transport Medium (Apsilon™ MTM)

• Inactivates viruses within seconds and bacteria within minutes.

• Preserves RNA (3 months) and DNA (18 months) at room temperature.

• Ethanol-free and fully compatible with all Apsilon™ extraction kits.

• Ensures safe sample transport without refrigeration.

• Demonstrated broad compatibility with downstream assays and sequencing.

NGS-Focused Capabilities

• Superior microbial diversity recovery vs leading kits (higher Shannon & Gini-Simpson indices).

• Better detection of Gram-positive species and broader taxonomic coverage.

• High Q20/Q30 sequencing quality.

• Suitable for metagenomics, 16S/ITS sequencing, and cfDNA/miRNA workflows.